cm1850 manual

cm1850 manual

Added all items to the cart. An error occurred while trying to add the items to the cart. Continue Shopping Proceed to Checkout Close Continue Shopping Proceed to Checkout Close Please upgrade to a modern browser. Contact us for any other documents regarding this product. Read carefully prior to operating the instrument! Leica reserves the right to change technical Heidelberger Str. 17 - 19 specifications as well as manufacturing pro- D-69226 Nussloch cesses without prior notice. The instrument has been designed for rapid freezing and sectioning of tis- sue samples. Prior to manipulating the knife and specimen, or changing the specimen or knife, and during breaks, always lock the handwheel.Only shut the tap when draining the waste container.Type CM 1850-11 CM 1850-03 CM 1850-07 CM 1850-08. Schurter, Typ FST; 6,3x32 mm oder T0,25 A Fa. Wickmann, Typ 19343; 6,3x32 mm F2: T0,6 A Fa. Schurter, Typ FST; 6,3x32 mm oder T0,6 A Fa. Wickmann, Typ 19343; 6,3x32 mm F3: T1,6 A Fa. Should you find any discrepancies, please contact your Leica sales office without delay. A choice of different knife holders is available for the CM 1850. The knife holder is accompanied by its own separate instruction manual. To turn the automatic mains fuse on, the switch must be set in the upper position (pos. 1). To turn the automatic mains fuse off, the switch must be set in the lower position (pos. When pushing the button for more than 1 second, the time value increases or decreases continuously (autorepeat function).Touch the button for indication of the beginning of the defrost cycle which has actually been set. At the same time, the LEDs between the indi- cation of hours and minutes are flashing. However, it is not possible to defrost both systems si- multaneously.During the defrost cycle, the indication is flashing. This is a safety feature to protect both the specimen and knife from damage.The temperature of the quick freeze shelf is always lower than the cryochamber temperature.

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The evaporator is flushed with hot gas dur- ing defrosting. The cryochamber virtually is frostfree and does not need to be defrosted. The condensation water that is produced during defrosting is collected in a container, which is located at the front of the cryostat cabinet. Please note, however, that it may take up to several hours to cool the cryochamber down to very low temperatures after turning the instrument on again. If the instrument fails to turn on, make a service call.The cryostat has to be disinfected after each daily use. Comply with the instructions for use. The glass anti-roll plate can remain in place during disinfection. Repairs may only be carried out by qualified service engineers autho- rized by Leica. The microtome can be removed for thorough cleaning and disin- fecting, or for extensive drying after a long power failure! 9.3.2 Removal of the microtome. After repeatedly drying the microtome in this manner, it may become neces- sary to relubricate the cross roller. Only use fuses of the same specification. The use of fuses other than specified by the manufacturer may cause severe damage to the in- strument. On the back of the instrument there is a fuse box. If an instrument or any part of it is to be returned to Leica, please note the following: a. If the instrument or any part of it has been exposed to or been in contact with potentially pathogenic or radioactive materials, it is essential to de- contaminate the instrument or part. Sign in Forgot Password. My Bench Close Sign In Not A Member. Sign Up Join MedWrench OK name type Receive Summary Emails. Leica Biosystems - CM1850 by Leica Biosystems Download PDF Product Details Forums Documents Videos News Request a quote Request Parts Add to My Bench This site uses cookies. By continuing to browse the site you are agreeing to our use of cookies. Please review our Privacy Policy for more details. All Rights Reserved. Sign Up Join MedWrench OK name type Receive Summary Emails.

This will also help other community members who face similar challenges. It monitors the heat of the line. The temp sensor needs replaced. Tech at Leica can assist you with the part. Dubyaj Report Post Hawkeye Biomedical LLC Sun Feb 24 2013 Reply from Hawkeye Biomedical LLC I am in need of a parts list. Report Post marilynmiller Sat Feb 14 2015 Reply from marilynmiller Report Post ram chandra Sun Jul 24 2016 Reply from ram chandra By continuing to browse the site you are agreeing to our use of cookies. Read carefully prior to operating the instrument. Page 2 Page 3 INFORMATION The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge and stateof-the-art technology as we understand it following thorough investigation in this field. We are under no obligation to update the present manual periodically and on an ongoing basis according to the latest technical developments, nor to provide our customers with additional copies, updates etc.Qualification of personnel The Leica CM1850 UV may only be operated by trained laboratory personnel. Page 7 2. Safety This instruction manual includes important instructions and information related to the operating safety and maintenance of the instrument. The instruction manual is an important part of the product which must be read carefully before setting up and using the instrument and must always be kept with the instrument. Page 8 2. Safety 2.1.2 Knife guard For every manipulation in the cryochamber, or upon changing a specimen when the knife or disposable blade is clamped, or during breaks, cover the cutting edge with the knife guard. Type Mark of conformity Nominal voltage Nominal frequency Power input Max.Please note that the compressor requires a start-up current between 45 and 50 A.

Therefore, the electric circuit at the installation site must be inspected by an electrical engineer to ensure that it meets the requirements for a smooth operation of the instrument. A constant adequate power supply to the instrument must be ensured at all times. Page 18 5. Setup 5.3 Leica CM1850 UV - Overview 17 15 4 13 16 21 20 8 13 13 19 12 10 18 14 9 Fig. 6 18 11 Instructions for use V 1. Page 19 5. Page 20 5. Setup 5.4 Mains switch and automatic mains fuse The automatic mains fuse is used as mains switch. Manual defrost button To activate and deactivate manual defrosting. Key button To lock and unlock the control panel to protect the entered parameters from unintended modifications. To lock or unlock, hold down for approximately 5 seconds. Page 22 6. Control panel operation 6.2 Programming the desired values 6.2.1 Setting the time The actual time is set on the panel marked with the clock symbol using the and keys. When pushing the or button for more than 1 second, the time value increases or decreases continuously (autorepeat function). Fig. 9 6.2.2 Setting the automatic defrost time (cryochamber) Fig. 10 The automatic defrost cycle takes place once within 24 hours. Page 23 6. Control panel operation 6.2.4 Activation of the Peltier element (option) Fig. 12 The Peltier element is used for cooling the quick-freeze stations. Upon activation of the Peltier element, the compressor of the cooling system is started after 40 seconds to reinforce the thermal conductivity effect. During the defrost cycle, the indication is flashing. LED (1) flashes, while the specimen head is in motion. Make sure that the shaft of the specimen disc is fully inserted. Page 28 7. Daily use of the instrument 7.3 Sectioning All necessary adjustments on the knife holder and anti-roll guide are described in the separate instruction manual for your knife holder in detail. 7.3.1 Trimming the specimen Take care when handling microtome knives and disposable blades.

The evaporator is flushed with hot gas during defrosting. The condensation water that is produced during defrosting is collected in a container, which is located at the front of the cryostat cabinet. Page 31 7. Daily use of the instrument 7.5.3 Manual defrosting of the quick freeze shelf The quick freeze shelf may become hot during defrosting. Therefore, do not touch it. Please note, however, that it may take up to several hours to cool the cryochamber down to very low temperatures after turning the instrument on again. The following error messages might occur during operation: Fig. 20 Error Description Remedy 20 Calibrating error; possibly defective controller board. 21 22 23 Clock battery on the controller board empty. Sections curl on the anti-roll plate - Anti-roll plate does not protrude far enough beyond the cutting edge. - Readjust correctly. Page 36 8. Troubleshooting Problem Cause Remedy - Knife profile inappropriate for the specimen to be cut. - Incorrect clearance angle. - Use knife with different profile. Anti-roll plate damage after adjustment - Plate too high above the cutting edge. For easy-to-use spray disinfection we recommend Leica Cryofect. (Cryofect is not available in all countries!) The cryostat has to be disinfected after each daily use. After repeatedly drying the microtome in this manner, it may become necessary to relubricate the cross roller bearings. The specimen head remains in the lower position. Page 46 9. Cleaning, disinfection, maintenance 9.3.5 Replacement of the fuses Turn the instrument off with the automatic mains fuse and pull the mains plug, before replacing the fuses. Only use fuses of the same specification. The use of fuses other than specified by the manufacturer may cause severe damage to the instrument. The scope of the warranty is based on the content of the concluded agreement. Page 53 12.

Decontamination Certificate (master) Dear Customer, any product that is to be returned to Leica Biosystems or serviced on site, must be cleaned and decontaminated in the appropriate manner. Important - to avoid refusal of shipment: Place one copy in the unit prior to packaging or hand it over to the service engineer. Contact us today for pricing and more information. A new insulation technology leads power savings of approximately 10% compared to conventional heat insulation systems. This new feature enhances the durability of the refrigerating system and ensures stable cryochamber temperatures, even when operating in unfavorable environmental conditions in the lab such as high humidity. Phone This field is for validation purposes and should be left unchanged. Cryostat. Instruction Manual. Always keep this manual near the instrument! Read carefully prior to operating the instrument!For erroneous statements, drawings, technical illustrations etc.In particular, no liability whatsoever is accepted for any financial lossStatements, drawings, illustrations and otherThese are determined only by the contract provisions agreed between ourselves and ourThis document is protected under copyright laws. Any copyrights of this document are retained by. Leica Microsystems Nussloch GmbH. Any reproduction of text and illustrations (or ofMicrosystems Nussloch GmbH. For the instrument serial number and year ofLeica Microsystems Nussloch GmbH. Heidelberger Str. 17 - 19. D-69226 Nussloch. Germany. Phone. Fax:Internet:Unpacking and installation. 14Prior to operation. 17. Leica CM1850 - Overview. 18. Mains switch and automatic mains fuse. 20. Turning the instrument on. 20. Control panel operation. 21Transport to the desired location. 14. Standard delivery. 15. Handwheel assembly. 16. Control panel 1. 21. Programming the desired values. 21. Setting the time. 21. Setting the automatic defrost time (cryochamber). 22. Selecting the cryochamber temperature. 22.

Activation of the Peltier element (option). 23. Manual defrosting of the quick freeze shelf. 23. Manual defrosting of the cryochamber. 24. Display lock. 24. Control panel 2 - Electric coarse feed. 25. Table of contentsSpray disinfection with Leica Cryofect. 40. Maintenance. 41. General maintenance. 41. Removal of the microtome. 42. Removal of the microtome cover. 44. Reinstallation of the microtome. 44. Replacement of the fuses. 46. Replacement of the lamp. 46. Ordering information, optional accessories. 48Temperature control button. 33. Possible causes and remedies. 34. Cleaning, disinfection, maintenance. 39Quick freeze shelf. 26. Specimen discs. 27. Inserting the specimen discs in the specimen head. 27. Specimen orientation. 27. Sectioning. 28. Trimming the specimen. 28. Section thickness setting. 28. Defrosting. 30. Automatic defrosting of the cryochamber. 30. Manual defrosting of the cryochamber. 30. Manual defrosting of the quick freeze shelf. 31. Terminating work. 31. Terminating daily work. 31. Shutdown for a longer period. 32. Ordering information. 48. Optional accessories. 49. Mobile heat extractor. 49. Thermal block. 49. Warranty and service. 50WarningsFigures in brackets refer to item numbers in drawings or to the drawingsThe Leica CM1850 may only be operated byAll laboratory personnel designated to operate the instrument must carefully read theDesignated use. The Leica CM1850 is a powerful cryostat forThe instrument has been designed for rapidThe instrument has not been designed for unattended storage of tissue material. The instrument may only be operated withinAny other use of the instrument is consideredAll information given in this instruction manualA name plate, indicating the instrument serialFig. 1This instruction manual includes important instructions and informationThe instruction manual is an important part of the product.

It must beIf additional requirements, which exceed the scope of this manual, areRead this instruction manual carefully before attempting to use or operate the instrument.The instrument and its accessory equipment incorporate the followingThe consistent use of these safety features and strict observation of thePrior to manipulating the knife and specimen, or changing theFor locking the handwheel rotate the handle until it is in the upper position. Push the locking pin (1) into the recess at the handwheel. The locking position is marked by a black dot (2). If necessary, move the handwheel slightlyTo unlock, push the locking pin (1) to the left from the recess at the handwheel. Fig. 3For every manipulation in the cryochamber, or upon changing aThe knife holders CN and CS are equipped with knife guards; on the knifePrior to manipulating the knife and specimen, or changing the specimenThis instrument has been built and tested in accordance with the following safety regulations on electrical measuring, control, regulating andTransport and installationConnection to mainsThe compressor requires a start-up current between 45 and 50 A. Therefore, the electric circuit at the place of installation must be inspected by an electrical engineer to ensure that it meets the requirements for a smooth operation of the instrument. A constant adequate power supply to the instrument must be ensured at all times. Failure to comply with the above will cause severe damage to theThis waiting period is necessary to allow the compressor oil, whichFailure to comply with this can cause severe damage to the instrument. Defrosting. Therefore, do not touch it!The cutting edge is extremely sharp and can cause severe injury!Only shut the tapThe instrument is appropriate for spray disinfection with Leica. Cryofect!Only use the cleaning agents and disinfectants specified in this instruction manual such as Leica Cryofect (alcohol or common disinfectants based on alcohol)!

Extended skin contact with cold parts of the instrument may causeReplacement of the fusesFor the required values. The use of fuses other than specified by the manufacturer may causeReplacement of the lampTypeNominal frequency. Power draw. Max. start-up current for 5 sec. Protective class. Automatic mains fuse. Pollution degree. Overvoltage installation category. Heat emission (max.)Cryochamber. Temperature setting range. Refrigeration capacity. Safety factor. Refrigerant. Compressor oilMax. temperature. Number of quick freeze stations 10. DefrostingPeltier element (option). Max. temperature. Number of freezing stationsMicrotome. Rotary microtome. Section thickness setting. Specimen feed. Vertical stroke. Maximum specimen size. Specimen orientation. Electric coarse feedFluorescent lamp. Color: LUMILUX light white. Color: LUMILUX light whiteCryocabinet. Dimensions. Width (with handwheel). Depth. Height. Weight (incl. microtome,All CM 1850 types require the following fuses. F1: T0,25 A Fa. Schurter, Typ FST; 6,3x32 mm. F2: T0,6 A Fa. Schurter, Typ FST; 6,3x32 mm. F3: T1,6 A Fa. Schurter, Typ FST; 6,3x32 mm. F4: T6,25 A Fa. Schurter, Typ FST; 6,3x32 mmFa. Fa. Littlefuse. Typ. TypDo not operate in rooms with explosion hazard!To ensure an adequate cooling capacity, the instrument must be set up with atHigh room temperatures and excessive air humidity affect theLengthwiseTiltThis is necessary to ensure stability. The instrument must be transported in an upright position only. When the instrument is tilted, the compressor oil is displaced. Do not grip the cabinet at the lid. Grip the cabinet only at theThe alignment of the adjustable feet is necessary to ensure anIt gives following variations:Handwheel, assy.Optional clamping lever for object head. 0112 28806. Specimen discs, 25mm diameter. 0416 19275. Section waste trayBottle of cryostat oil, type 407, 50 ml. 0336 06098. Bottle of OCT-Compound, mounting medium for cryosectioning, 125 ml. 0201 08926.

Compare the delivered components with the parts list and your order. Should you find any discrepancies, please contact your Leica sales office without delay. The knife holder is accompanied by its own separate instructionPlease contact your Leica sales office if the instruction manualTo dismount, proceed in reverse order. Fig. 5. The handwheel including the fixing components are packed in theConnection to mains power. During the start-up of the compressor the nominal voltage must not. Please note that the compressor requires a start-up current between 45 and 50 A. Therefore, the electric circuit at the installation site must be inspected by an electrical engineer to ensure that it meets the requirements for a smooth operation of the instrument. Failure to comply with the above will cause severe damage to theDo not connect any other consumers to this electric circuit. Prior to operation. Before connecting the instrument to the mains power, please check ifPlace the storage shelves in the cryochamber. Place the section waste tray and brush shelf in the cryochamber. Place the knife holder base on the microtome base plate. Insert the knife holder and clamp it on the base plate For details, pleaseOpen the knife box with the knife and place it in the cryochamber forPlace all tools needed for specimen preparation in the cryochamber. Close the sliding window. Connect the mains plug to the mains power outlet at the wall.Waste container. Control panel 1. Control panel 2. Storage shelf, left. Storage shelf, right. Support for stationary heat. Quick freeze shelf. Peltier element (accessory). Stationary heat extractor. Specimen disc. Thermal bloc (accessory). Section waste tray. Brush shelf. Orientable specimen head. Knife holder base (accessory). Knife holder CE (accessory). Knife holder CN (accessory). Knife holder CS (accessory)The automatic mains fuse is used as mains switch.

To turn the automatic mains fuse on, the switch must be set in the upperTo turn the automatic mains fuse off, the switch must be set in the lowerFig. 7. Automatic mains fuseAfter transporting, wait at least 4 hours before turning the instrument on. This waiting period is necessary to allow the compressor oil,Failure to comply with this can cause severe damage to the instrument. Time. Defrost time. Cryochamber cooling. Peltier element (option):Off. During normal operation pressure compensation prior to the compressor start-up might lead to a hissing sound.Fig. 8. Function keys. Lamp button. Manual defrost button. To activate and deactivate manual defrosting. Key button. To lock and unlock the control panel to protect the entered parameters fromTo lock or unlock, hold down for approximately 5The actual time is set on the panel marked with the clock symbol using theWhen pushing theFig. 9Fig. 10. The automatic defrost cycle takes place once within 24 hours. Touch the or button for indication of the beginning of the defrost cycleAt the same time, the LEDs between the indication of hours and minutes are flashing. To change the beginning of the defrost cycle in steps of 15 minutes, pushFig. 11The actual temperature is the standard indication. For indication of the desired value, touch the or button. Set the desired value with the and buttons. When pushing the orThe actual value will be indicated 5 seconds after finishing the programming.Fig. 12. The Peltier element is used for cooling the quick-freeze stations. Upon activation of the Peltier element, the compressor of the cooling system is startedDisplay reading of instrumentsThe Peltier element is activated by pressingThe Peltier element turns off automatically after 10 minutes.The quick freeze shelf may become hot during defrosting! Therefore, do not touch it!

The manual defrosting of the quick freeze shelf is activated by subsequentlyTo turn off the manual defrosting cycle of the quick freeze shelf prior to theQuick freeze shelf and cryochamber defrosting can be run independently. However, it is not possible to defrost both systems simultaneously.The manual defrosting of the cryochamber is activated by subsequentlyIf you want to turn off the manual defrosting of the quick freeze shelf prior toFig. 14The programmed values cannot be modified after having pushed the keyPush the key button once more for 5 seconds to unlock the display. When the display is locked, the LEDs between the hour and minute indication on the time panel are turned off. Fig. 15Move the specimen away from the knife. On reaching the rear limit, the LED (1) starts illuminating.The specimen will slowly move to the rear limit, as long as the button isMove the specimen towards the knifeOn reaching the front limit, the LED (2) of the button starts illuminating.The temperature of the quick freeze shelf is always lower than the cryochamberAfter approximately 30 seconds contact, the specimen will be entirely frozen.Make sure that the shaft of the specimen disc isFig. 18All necessary adjustments on the knife holder and anti-roll guideTake care when handling microtome knives and disposableReadjust the anti-roll plate if necessary.The selected section thickness is indicated on the index mark on theAfter changing from one section thickness to another, the first two or three sections shouldAdrenal. Bone marrow. Brain. Bladder. Breast - fatty. Breast - little fat. Cartilage. Cervical. Fatty. Heart and vascular. Intestinal. Kidney. Laryngeal. Lip. Liver. Lung. Lymphoid. Muscular. Nose. Pancreatic. Prostate. Ovarian. Rectal. Skin with fat. Skin without fat. Spleenal or bloody tissue. Testicular. Thyroid. Tongue. Uterus curettageDefrosting the cryochamber actually means defrosting the evaporator toThe evaporator is flushed with hot gas during defrosting.

The cryochamber virtually is frostfree and does not need toThe condensation water that is produced during defrosting is collected in aTo make sure that the condensation water stemming from the defrost cycles drains into the waste container and to avoid the risk ofThe quick freeze shelf is cooled during the automatic cryochamberCooling turns back on automatically.An automatic defrost cycle takes place once in 24 hours. The time of the automatic defrost cycle is programmed via the control panelIn addition to the programmable automatic defrost cycle a manual defrostTo avoid an unintended defrosting, the activation of a manual defrost cycle is confirmed by an audible signal. Cooling turns back on automatically.The quick freeze shelf may become hot during defrosting! If increased frost formation occurs on the quick freeze shelf, especially after spray disinfection, a manual defrost cycle should be started (see 6.2.5),Only common detergents and disinfectants that contain 95 - 98 %All components removed from the cold environment will collectRemove all specimens from the cryostat. Turn out the cryochamber illumination. Lock control panel 1 (Fig. 8) with the KEY button. Do not turn the instrument off with the automatic mains fuse as thereIf you do not intend to use the instrument for several weeks youPlease note, however, that it may take up to several hours to coolAfter turning off, the instrument should be cleaned and disinfected. Turn the instrument off with the automatic mains fuse. Open the sliding window to allow the cryochamber to dry. Remove all the specimens from the cryostat. Lock the handwheel. Put the knife back into theTurning off the instrument with the automatic mains fuse will not affect theBefore turning the instrument on again, the cryochamber microtome and allError messages are displayed on the clock panel as follows: EO: XX. TheFig. 20. Error. Description. RemedyMicrotome wet. Cryochamber temperature out of range of indication.

Short circuit at the temperature sensorBreaking of the temperature sensorShort circuit at the temperature sensor of the evaporator. Breaking of the temperature sensor of the evaporator. Turn the instrument on again. If the error is displayed again. Call service. Dry microtome. Remove cause.Call service. Temperature control button. On the back of the cryostat cabinet there is a temperature control button (1). If the temperature of the. Possible causes and remedies. Fig. 21After eliminating the possible source of error, push the temperature control button (1) to turn the instrument back on. If the instrument fails to turn on, make a service call.Problem. Cause. Remedy. Frost on chamber walls and microtomeIce formation on the bottom ofSections smearSections splinterSections not properly flattenedProblem. Sections not properly flattenedSections curl on the anti-rollScraping noise during sectioningRidged sectionsChatter during sectioningProblem. RemedyCondensation on anti-roll plateAnti-roll plate damage after adjustmentThe adjustment wasThick-thin sectionsTissue sticks or crumbles on theProblem. Fat on the corner or edge of theSpecimen disc cannot be removedCryostat inoperationalFlattened sections curl up whenSections tear. Inconsistent or insufficient specimen feedProblem. No or insufficient refrigerationScraping noise at the microtomeDo not use organic solvents or any other aggressive substances forOnly use the cleaning agents specifiedCondensation water produced during defrosting collects in theFor easy-to-use spray disinfection we recommend Leica Cryofect.