Agilent Dad Detector Manual

Agilent Dad Detector Manual

Need Repairs, maintenance or installation for your lab equipment. Connect with a community of owners and qualified service providers at LabWrench.com Visit LabWrench.com Find a Service Provider Join Product Communities Shop Brands Featured Brands See All Brands Shop by Brand Agilent Thermo Fisher Eppendorf VWR Metrohm Ohaus Waters Bruker Shimadzu Corning Huber Perkin Elmer Beckman Sciex Olympus Nikon Labconco Biotek Shop Applications Shop Applications Explore the latest products and resources related to your industry. Technological innovations, prominent manufacturers and popular equipment - all in one place. View All Applications Shop Re-Sellers Shop Featured Re-Sellers Shop All Stores Resources Resources, Guides and Articles Learn about equipment technologies and science in our resource center. Browse articles and infographics to get the latest industry insights. Topics Buying Guides Cannabis Laboratory Chromatography Infographics Mass Spectrometry Product Review Reasons to Upgrade Technical Insight View All Featured Infographics Featured Resources Auction Events Auction Events Check out upcoming equipment auctions on our event calendar. Score liquidation pricing on an incredible assortment of products. Connect with a community of owners and qualified service providers at LabWrench.com Visit LabWrench.com Find a Service Provider Join Product Communities Shop Brands Featured Brands See All Brands Shop by Brand Agilent Thermo Fisher Eppendorf VWR Metrohm Ohaus Waters Bruker Shimadzu Corning Huber Perkin Elmer Beckman Sciex Olympus Nikon Labconco Biotek Shop Re-Sellers Shop Featured Re-Sellers Shop All Stores Shop Applications Shop Applications Explore the latest products and resources related to your industry. View All Applications Resources Resources, Guides and Articles Learn about equipment technologies and science in our resource center.

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From time to time we also sell instruments, which always come fully refurbished, tested and performing within manufacturer's specifications. At high data rates, the 1200 Series DAD still provides precise identification, quantification, and peak purity analysis at trace levels. This exceptional performance is particularly useful when ultrafast LC methods must comply with strict regulatory requirements. The unit provides an expanded detection range, 190 nm to 950 nm, with the lowest detection limit. Low-noise front-end electronics and a new patented flow cell design deliver lowest detection limits as a result of minimized short-term noise. This detector will ship out with a data packet containing all the performance data from testing showing it works within all manufacturer specifications. We also buy, refurbish, and resell used lab equipment. Our customer base includes large multinational pharmaceutical companies, cutting edge biotech firms, preeminent research Universities, and US government research institutions. Designated trademarks and brands are the property of their respective owners. Use of this Web site constitutes acceptance of the LabX User Agreement. Diode Array and. Multiple Wavelength. Detector. Service ManualWarranty. No part of this manual may be reproduced inTechnologies, Inc. as governed by United. States and international copyright laws. The material contained in this document is provided “as is,” and is subject to being changed, without notice,Agilent shall not beAgilent and the user have a separateManual Part NumberPrinted in Germany. Agilent Technologies. Hewlett-Packard-Strasse 8Manual Structure. Technology Licenses. The Service Manual G1315-90112 (English)Multiple Wavelength Detector. It is available as Adobe Reader file (PDF) only.The User Manual G1315-90012 (English)Latest versions of the manuals can beSoftware and technical data rights grantedFAR 12.211 (Technical Data) and FAR 12.212Defense purchases, DFARS 252.227-7015DFARS 227.

7202-3 (Rights in Commercial. Computer Software or Computer Software. Documentation). If a federal government orSafety Notices. Refer also to “General SafetyIt calls attention to an operating procedure, practice, or the likeCAUTION notice until the indicatedThis manual contains information for using and servicing yourThis chapter gives an introduction to the detector, instrumentThis chapter provides information on environmentalThis chapter provides information on unpacking, checking onThis chapter provides information on connecting the detector toThis chapter provides information on how to set up the detectorThis chapter provides information on how to optimize theThis chapter gives an overview about the troubleshooting andThis chapter describes the meaning of detector error messages,This chapter describes the detector’s built in test functions.This chapter describes the detector’s built in diagnostic signals.This chapter provides general information on repairing theThis chapter describes the maintenance of the detector.This chapter describes the repair of the detector.This chapter provides information on parts for maintenance.This chapter provides information on cables used with the 1200This chapter describes the detector in more detail on hardwareAnnex A. Appendix. This chapter provides addition information on safety, legal andContentIntroduction to the Detector. Optical SystemInstrument LayoutSite RequirementsUnpacking the Detector 34. Damaged Packaging 34. Delivery Checklist 34. Optimizing the Stack Configuration. Installing the DetectorSetting up the LAN accessWhat you have to do firstConfiguring the Agilent Bootp service program. Storing the settings permanently with Bootp. Manual Configuration 62. With Telnet 63. With the Instant Pilot (G4208A) 67. With the Handheld Controller (G1323B)PC Setup for Local Configuration 71. Agilent ChemStation Setup 72Setting up an Analysis 76. Before Using the System 76. Requirements and Conditions 78.

Optimization of the System 80. Preparing the HPLC System 81. Running the Sample and Verifying the ResultsControl Settings 91. Configuration Settings 92. Online Spectra (DAD only) 93. Analog Output Settings 97. Spectrum Settings (DAD only) 98. Peakwidth Settings 100. Slit Settings 102. Margin for Negative Absorbance Settings 103. Optimizing the Detector 103Optimizing the Detector Performance. Optimization OverviewDispersion 109. Flow Cell Path Length 109. Peak width (response time) 111. Sample and Reference Wavelength and Bandwidth. Slit Width 115. Optimizing Spectral Acquisition (DAD only) 118. Margin for Negative Absorbance 119Quantifying Coeluting Peaks by Peak Suppression 120. Ratio Qualifiers for Selective Detection of Compound. Classes 122Overview of the Detector’s Indicators and Test FunctionsPower Supply Indicator 127. Detector Status Indicator 128. User InterfacesWhat Are Error MessagesTimeout 133. Shutdown 134. Remote Timeout 135. Synchronization Lost 136. Leak 137. Leak Sensor Open 138. Leak Sensor Short 138. Compensation Sensor Open. Compensation Sensor Short. Fan Failed 140. Open Cover 141. Cover Violation 142Visible Lamp Current 143. Visible Lamp Voltage 144. Calibration Values Invalid 145. Wavelength Recalibration Lost 146. Diode Current Leakage 147. Holmium Oxide Test Failed 148. UV Lamp Current 149. UV Lamp Voltage 150. UV Ignition Failed 151. UV Heater Current 152. Illegal Value From Temperature Sensor 153. Illegal Value From Air Inlet Temperature Sensor 154. Heater Failed 155. Heater Power At Limit 156. DSP Not Running 158Self-test. Filter Test. Slit TestIntensity TestASTM Noise Test. Cell TestAgilent ChemStation 179. Control Module G1323B 180. Diagnosis Information on Agilent ChemStationOverview of Diagnostic SignalsProblem Description 185. Problem Verification 185. Solution 186. Optimization Information 187. Examples of baselines 187Specifications and Conditions 191.

Principle of Temperature Control 192Start a Run and EvaluateIntroduction to Maintenance and Repair 202. Simple Repairs - Maintenance 202. Exchanging Internal Parts - Repair 202. Warnings and CautionsUsing the ESD StrapOverview of Maintenance. Exchanging a LampMaintenance of High Pressure Flow CellFlow Cell 227. Nano Flow Cell - Replacing or CleaningCorrecting LeaksIntensity Test 244. Wavelength Verification and Recalibration. Holmium Oxide Test 247. Cell Test 249Cautions and WarningsCleaning or Replacing Cell Support Window. Removing the Top Cover and FoamUsing the Agilent ChemStation 267. Using the Instant Pilot G2408A 268. Using the Control Module G1323B 270. Removing the PCMCIA Interface. Replacing Fan PartsRemoving the Optical UnitReplacing the Holmium Oxide Filter MotorLimiter 289. Exchanging the Power Supply. Exchanging the Leak SensorAssembling the Main CoverOverview of Maintenance Parts. Standard Flow Cell. Micro Flow CellPrep Flow Cell - SSTNano Flow Cells. Accessory KitParts At The Rear Of The Detector. Optical Unit AssemblyCell Support Assembly. Holmium Oxide Filter. Fan Assembly Parts. Plastic Parts. Leak PartsFoam PartsSheet Metal KitCable OverviewCAN CableRS-232 Cable Kit. LAN CablesMain features: 360. Firmware 361. Leak Sensor 361. Fan Drive 361. Analog Signal Outputs 361. Deuterium Lamp Filament and Current Control. Tungsten Lamp Voltage Control 362. Safety Switches 362. Interfaces 362. Temperature Control 362. Internal Clock without Battery 362. Lamp and Flow Cell RFID Tag 363PCMCIA Slot 364Interfaces 367. Analog Signal Output 368. LAN Interface 368. CAN Interface 368. Remote Interface 369LAN Settings 374. RS-232C Communication Settings. Boot-Resident Settings 374. Forced Cold Start Settings 375. Firmware Description. Firmware UpdatesGeneral Safety InformationRadio Interference. Sound Emission. UV-RadiationAgilent Technologies on InternetAgilent 1200 Series Diode Array and Multiple Wavelength Detector. Service ManualIntroduction to the Detector 16.

Optical System 17. Early Maintenance Feedback (EMF) 20. Instrument Layout 22. Electrical Connections 23. This chapter gives an introduction to the detector, instrument overview andAgilent TechnologiesIntroduction to the Detector. The detector is designed for highest optical performance, GLP compliance andFor specifications, see “Performance Specifications” on page 30.IntroductionThe optical system of the detector is shown in Figure 1. Its illumination sourceThe image of the filament of theThis allows simultaneous access to allCell supportTungsten lamp. Flow cell. Coupling lens. Spectro lens. Deuterium lamp. Slit. Diode array. Achromat (source lens). Holmium oxide filter. Grating. Figure 1. Optical System of the DetectorLamps. AchromatThe light source for the UV-wavelength range is a deuterium lamp with aAs a result of plasma discharge in low-pressureThis lamp emits light over the wavelength. The achromat receives the light from both lamps and focuses it so that theHolmium Oxide. Filter. The holmium oxide filter is electromechanically actuated. During the holmiumCell Support. Window. The cell support window assembly separates the holmium filter area from theFlow Cell. Compartment. The optical unit has a flow cell compartment for easy access to flow cells. ASpectrograph. The spectrograph material is ceramic to reduce thermal effects to a minimum. The spectrograph consists of the spectrograph lens, the variable entrance slit,See note on page 32. Wavelength accuracy. Self-calibration with deuterium lines,Wavelength bunching. Programmable in steps of 1 nm. Slit widthProgrammable slit. Diode widthFigure 20. Telnet - Starting a sessionWhen the connection was established successfully, the detector respondsFigure 21. A connection to the module is madeFigure 22. Table 9. Telnet Commands. Value. DescriptionLAN ConfigurationMAC address, initialization mode. Initialization mode is Using StoredChemStation), here not connected. Figure 23.

Telnet - Current settings in “Using Stored“ modeInitialization mode is Using StoredChemStation), here not connected. Figure 24. Telnet - Change IP settingsFigure 25If the Initialization Mode Switch is changed now to “Using Stored” mode, the instrumentIn the example above it wouldLAN ConfigurationFigure 26. Instant Pilot - LAN ConfigurationWith the Handheld Controller (G1323B)Figure 27. Select moduleFigure 28LAN ConfigurationThe informationFigure 29. Diode Array and Multiple Wavelength Detector Status Page. In Figure 30 on page 69 the complete listing is shown. For explanations referLAN Status PageBootp Server: 134.40.29.56Figure 30. LAN interface Status Page (complete)Figure 31LAN ConfigurationPC Setup for Local ConfigurationFigure 32Agilent ChemStation SetupFigure 33LAN ConfigurationChemStation.Agilent 1200 Series Diode Array and Multiple Wavelength Detector. Service ManualSetting up an Analysis 76. Running the Sample and Verifying the Results 90. Special Settings of the Detector 91. Control Settings 91. Optimizing the Detector 103. This chapter provides information on how to set up the detector for anAgilent TechnologiesSetting up an Analysis. This chapter can be used forIt is not a test of theBefore Using the System. Solvent Information. Observe recommendations on the use of solvents in chapter “Solvents” in thePriming and Purging the System. When the solvents have been exchanged or the pumping system has beenSolvents containing volatileTherefore priming of the pumping systemUsing the Detector. Table 10Activity. Solvent. Comments. After an installation. Isopropanol. Best solvent to flush air out of theWhen switching between reverseIsopropanol. Ethanol or Methanol. Best solvent to flush air out of theTo clean the system when using buffers Bidistilled water. After a solvent change. Alternative to Isopropanol (secondBest solvent to re-dissolve bufferBidistilled water.

Best solvent to re-dissolve bufferAfter the installation of normal phaseThe pump should never be used for priming empty tubings (never let the pump run dry). UsePump for approximately 10 minutes before starting your application.Requirements and Conditions. What You Will Need. Table 11 lists the items you need to have for the set up of the analysis. Some ofTable 11. What you will needPump (plus degassing). Autosampler. Detector, standard flow cell installedSystem should be correctly set up for LAN communication with the Agilent. ChemStationZorbax Eclipse XDB-C18, 4.6 x 150 mm, 5 um. Part No. 993967-902 or Part No. 5063-6600. Standard. Part No. 01080-68704Using the DetectorA single injection of the isocratic test standard is made under the conditionsTable 12. Conditions. Flow. StoptimeSolvent. Temperature. Ambient. WavelengthInjection VolumeTypical Chromatogram. A typical chromatogram for this analysis is shown in Figure 34. The exactVariations in solvent quality, column packing, standard concentration andFigure 34. Typical Chromatogram with UV-detector. Optimization of the System. The settings used for this analysis are specific for this purpose. For otherPlease refer to theUsing the DetectorIf the pump, autosampler,ChemStation screen should look like shown in Figure 35. The System status is red (Not Ready). System status. On-line plot window. Figure 35Initial ChemStation screen (Method and Run Control)On button or the buttons below the module icons on the graphical userFigure 36Using the DetectorSystem” on page 76.Otherwise the detector baseline may still drift (depending on the environment). See also. Figure 37. Table 13. Stabilization of Baseline (both lamps turned on at the same time). Baseline drift after lamp turn on (example from Figure 37)Press OK to leave this screen. Figure 43. Sample InformationRunning the Sample and Verifying the ResultsChemStation will show the resulting chromatogram.

Figure 44Information about using the Data Analysis functions can be obtained from the Using your. ChemStation manual supplied with your system.Using the DetectorControl SettingsSettings” on page 97.Figure 45. Detector control settingsConfiguration SettingsRFID tags. If no RFID tag lamp isRFID tags. If no RFID tag cell is used,The detector status shows “Cell-tag” inRFID tag is not inserted. The detectorFigure 46If the flow cell temperature is critical for your chromatography, you may set the. Temperature Control to off. This will lower the optical unit and flow cell temperature byUsing the DetectorFigure 47. Online Spectra WindowThe detector’sThe detector supports run buffering, which means that an amount of run dataIf there is a temporary network failure or the PC is not able to constantly takeIf there is a permanent network failure, the Run Recovery dialog boxA sequence will be stopped in case of a network problem.In this case refer to “No RunUsing the DetectorTable 14. Automated Run Recovery in case of temporary communication failures. Situation. Reaction. All OK. Run in progress - Data Analysis. Elapsed run time is running. Spectra counter is running. Data are stored on PC and on card. LAN breaks. Error Power Fail. Elapsed run time stops. Spectra counter stops. Data continues to be stored on card. LAN recovers. Error Power Fail cleared. Elapsed run time continues at actual time. Spectra counter continues. Data continues to be stored ion PC and on card. ChemStation tries already to add missing dataStop time elapsed. ChemStation continues to ads missing data. Run endsIf the detector status window is not opened, you will realize only the Power Fail error andManual Run Recovery in case of permanent communication failuresStart a recoverFigure 48In this case refer to “No RunUsing the DetectorTo change the Output Range of the analog outputs see “Control Settings” onZero Offset Limits: 1 to 99% in steps of 1%. Attenuation Limits: 0.98 to 2000 mAU atFigure 49.

Analog Output SettingsSpectrum Settings (DAD only). To change the Spectra settings open.Table 15 on page 99 shows the possible parameters.Figure 50Using the Detector. Table 15Store. Defines at which points on “signal A” spectra will be taken and saved. Signal A is used to control the “peak controlled spectra acquisition”; theNone. No spectra are taken. Spectra are taken at the apex and baselines of the peak. Baselines. Spectra are taken at the apex, baselines, upslope, and downslope of theAll in Peak. All spectra within the peak are taken.The three spectra acquisition types mentioned above are also referred toThe peak detection is done by theThe integration algorithm also includes peak detection based on theEvery 2nd spectrum. Spectra are taken continuously as for All, but only every second spectrumThis reduces the amount of dataAll. Spectra are taken continuously depending on the setting of the. Peakwidth. Eight spectra are acquired per Peakwidth. The acquisitionIf there are no peaks in Signal A, there are no spectra. You cannot processRange. Range defines the wavelength range for spectral storage. Limits: 190 to 950 nm in steps of 1 nm for both low and high values. TheStep. Step defines the wavelength resolution for spectral storage.